Luciferase Arabidopsis Assay

Instruments

 

Cell Physiology in Multiwell Plates
Improvised heat  keeps  cells happy

Resources can be booked by the hour, using the KEENSCOPES group name, on the Faces booking site (faces.ccrc.uga.edu).

Do not use between 1am and 3am without arranging for the Ozone room sterilizer to be switched off.

Faces booking Site


There are three confocal microscopes, and a confocal-ready combination microscope.

Cancelling a booking on the confocal systems needs 24h notice, to give other users a chance to arrange use of the vacated time on these busy systems.  All other resources can be canceled up to the start of the first booked hour.


 

Instrument

Room

Description

Zeiss 880 Inverted 1002 Highest performance inverted spectral UV Airyscan Fast with incubator
Elyra PALM 1002 Add-on module to Zeiss inverted for super-resolution and TIRF
Leica SP5 1008 User friendly 5 channel spectral confocal
Zeiss 880 Upright 1004 Most sensitive upright Airyscan Fast
Keyence BZ-X710 1016 All-in-one inverted fluorescence scope with tiling  and extended depth

Other imaging equipment includes the latest Hitachi environmental SEM; a luminescence imager with fluorescence capability, which had new filters added for a 2017 Nature Methods paper,   a high performance fluorescence stereo microscope, an enlarger stand with camera, and a small LED equipped stereo scope for viewing only.  A Singer Mk1 manual micro manipulator can be used with the stereo microscope for fine dissection work.

 

Instrument

Room

Description

Hitachi TM4000PlusE II 1006 Environmental SEM for live and frozen samples
Luminescence Dark Box 1015 PIXIS 1024B camera on Stanford Photonics enclosure
Leica MZ FLIII  1006 Stereo scope with 24MP Canon M50 tethered camera
Nikon Multiphot 1007 Enlarger stand with PC-controlled retail camera
EMS Nightsea Stereo Scope 1005 Simple LED-fluorescent scope for viewing only


For sample preparation, there is a cryostat, oscillating tissue slicer (Vibratome) and ultramicrotome, a gene gun, a laminar flow hood, an incubator for animal cells, a vacuum oven, a manual micromanipulator, and an Arabidopsis growth chamber:  Also a critical point dryer, and a sputter coater with Gold Palladium substrate.  Our microtome is moving to the Histology Core in Webber Hall.

 

Instrument

Room

Description

Cryostat Hacker Bright 1005 Motorized Cryostat cuts up to 300um
EMS5000 1005 Oscillating tissue slicer
EMS 850 1005 Critical point dryer for SEM preparation
Quorum SC7620 1005 Au/Pd Sputter coater
Ultracut T 1015 Ultramicrotome with cryo capability
Vacuum Oven 1015 For plastic embedding
Hacker 5030 1006 Microtome for routine plastic/wax sectioning
PDS1000/He 1005 BioRad gene gun with vacuum chamber
Singer Mk 1 1006 Micromanipulator for fine dissection
Clean bench 1005 Labcono 5 ft horizontal Laminar flow hood
37° incubator 1017 For short-term animal cell storage
Growth Chamber Hall 25°C, 24h daylight, 8" shelves


The large dual-bridge robot has a sterile enclosure and high-volume plate carousel.  This system is functional but no longer has service support.

 

Instrument

Room

Description

Biomek FXP 1016 96 & span-8 robot with Cytomat & sub-µl pin tools


Imaris by Bitplane is a very powerful 3D and 4D data display and analysis package, running on a very fast PC, which can be operated remotely. Zen3 is Zeiss software which can be used for processing Airyscan and Airyscan Fast datasets.  ZigZag is tiling software, used on the new Hitachi TM4000ePlus, which then uses Microsoft ICE to stitch together very large electron micrographs.  Many users like to use the free FIJI or Image J software to do post-processing on their own computers.

 

Instrument

Room

Description

Imaris Bitplane 1007 User Friendly 3D rendering software
Zen3 1004,1007 Zeiss Analysis Software
Microsoft ICE 1006 Tiled SEM image assembly

 

Inverted Confocal with Incubator
Our Most Popular Microscope is the Zeiss880 Inverted Airyscan Fast

 

 

 

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